Journal: Biomolecules

Article Title: Production, Purification, and Characterization of a Novel Cysteine-Rich Anticoagulant from the Medicinal Leech and the Functional Role of Its C-Terminal Motif

doi: 10.3390/biom15121633

Figure Lengend Snippet: Content of E. coli proteins, DNA, and lipopolysaccharides (endotoxins) in purified CRA samples.

Article Snippet: The resulting mixture, without ligation, was used to transform Escherichia coli TOP10 cells (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), which were plated on LB agar plates containing ampicillin (150 mg/L).

Techniques: Purification